The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WNT2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)85-105%90-105%86-103%91-105%EDTA plasma(n=5)85-100%84-100%89-99%82-99%heparin plasma(n=5)84-99%82-99%86-97%88-99%
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Matrices listed below were spiked with certain level of WNT2B and the recovery rates were calculated by comparing the measured value to the expected amount of WNT2B in samples.MatrixRecovery range(%)Average(%)serum(n=5)89-10596EDTA plasma(n=5)87-10495heparin plasma(n=5)87-9693