Enter to Debug Area Tumor Necrosis Factor Beta ELISA Kit BHE10101056

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Tumor Necrosis Factor Beta ELISA Kit BHE10101056

SKU : BHE10101056

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appl-description-1	Double-antibody Sandwich
targ-gene-id-1	Reagents Quantity Reagents QuantityPre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4Standard 2 Standard Diluent 1×20mLDetection Reagent A 1×120µL Assay Diluent A 1×12mLDetection Reagent B 1×120µL Assay Diluent B 1×12mLTMB Substrate 1×9mL Stop Solution 1×6mLWash Buffer (30 × concentrate) 1×20mL Instruction manual 1
targ-pathway-1	15.6-1000pg/mL
prop-purity-1	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tumor Necrosis Factor Beta (TNFb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)88-99%85-96%80-89%92-103%EDTA plasma(n=5)86-101%93-101%78-92%99-105%heparin plasma(n=5)82-103%84-103%96-103%85-93%
appl-applications-1	3h
refe-reference-1:{74}(References) Reference	< 6.4pg/mL
manual-link-0:{925}(Protocols) Product Manual	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Beta (TNFb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
manual-link-3	Matrices listed below were spiked with certain level of recombinant Tumor Necrosis Factor Beta (TNFb) and the recovery rates were calculated by comparing the measured value to the expected amount of Tumor Necrosis Factor Beta (TNFb) in samples.MatrixRecovery range (%)Average(%)serum(n=5)78-9994EDTA plasma(n=5)80-9289heparin plasma(n=5)86-10399
prot-url-1	Biological Fluids, Cell Culture Supernates, Cell Lysates, Tissue Homogenates, Plasma, Serum
prop-storage-buffer-1	Biological Fluids, Cell Culture Supernates, Cell Lysates, Tissue Homogenates, Plasma, Serum

desc-gene-alt-name-15	Oryctolagus cuniculus (Rabbit)

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SKU	Supplier No.	Size	Your price	Quantity
BHE10101056		96T	$649.80		Add to Cart

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