The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TMEM132D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)87-99%95-105%88-101%87-103%EDTA plasma(n=5)86-101%87-100%82-100%85-94%heparin plasma(n=5)81-100%85-94%81-96%83-100%
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Matrices listed below were spiked with certain level of TMEM132D and the recovery rates were calculated by comparing the measured value to the expected amount of TMEM132D in samples.MatrixRecovery range(%)Average(%)serum(n=5)87-9792EDTA plasma(n=5)89-10398heparin plasma(n=5)89-10295