The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Septin-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)85-103%90-103%85-102%89-102%EDTA plasma(n=5)82-91%82-98%84-100%84-99%heparin plasma(n=5)84-100%85-99%81-87%80-95%
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Matrices listed below were spiked with certain level of Septin-5 and the recovery rates were calculated by comparing the measured value to the expected amount of Septin-5 in samples.MatrixRecovery range(%)Average(%)serum(n=5)86-9993EDTA plasma(n=5)88-9792heparin plasma(n=5)92-10495