The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEMA3F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)92-103%87-103%85-105%86-101%EDTA plasma(n=5)84-93%84-96%84-98%82-95%heparin plasma(n=5)88-99%83-96%81-95%82-100%
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Matrices listed below were spiked with certain level of SEMA3F and the recovery rates were calculated by comparing the measured value to the expected amount of SEMA3F in samples.MatrixRecovery range(%)Average(%)serum(n=5)86-10491EDTA plasma(n=5)90-10497heparin plasma(n=5)86-10192