Enter to Debug Area Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

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Home / Assay Kits

Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

SKU : BHE13502340

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Wuxi Donglin Sci & Tech Development Co.,Ltd.

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targ-target-details-1	The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.
desc-backbone-size-w-o-insert-15	The microtiter plate provided in this kit has been pre-coated with an antibody specific to GCLC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GCLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GCLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GCLC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
desc-5-cloning-site-15	Traditional ELISA KitReady-to-Use ELISA KITProduct name:Rat Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA KitMethod: SandwichSynonyms:GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECSDetection range:0.78 -50ng/mLReactivity: GCLCSize:96T/48TQuality guarantee period:for 12 monthsCatalog number:DL-GCLC-Ra(traditional)DLR-GCLC-Ra(ready-to-use)Assay length 1-4.5Hours 1-3.5HoursAdvantages:Competitive price.High sensitivity.High stability.12 months shelf life.Pre-diluted Detection Reagent A and BReduction in the number of steps when conducting the testAll the reagents can be stored at 4℃Faster reaction compare to other brands12 months shelf lifeInstruction Manual
manual-link-3	Matrices listed below were spiked with certain level of recombinant GCLC and the recovery rates were calculated by comparing the measured value to the expected amount of GCLC in samples.MatrixRecovery range (%)Average(%)serum(n=5)87-10194EDTA plasma(n=5)90-10195heparin plasma(n=5)91-10296
manual-link-0:{925}(Protocols) Product Manual	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100Intra-assay: CV
prop-purity-1	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCLC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1：21：41：81：16serum(n=5)82-96%83-98%81-99%93-101%EDTA plasma(n=5)88-101%86-95%90-102%80-93%heparin plasma(n=5)80-91%82-90%95-104%79-95%
prop-description-1	ItemStandardTestDescription The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GCLC in rat serum, plasma, tissue homogenates and other biological fluids.ConformIdentificationColorimetricPositiveCompositionTraditional ELISA KitReady-to-Use ELISA KITConformPre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1Plate sealer for 96 wells 2Plate sealer for 96 wells 2Standard 2Standard 2Diluents buffer 1×45mLStandard Diluent 1×20mLDetection Reagent A 1×120μLDetection Solution A 1×12mLDetection Reagent B 1×120μLDetection Solution B 1×12mLTMB Substrate 1×9mLTMB Substrate 1×9mLStop Solution 1×6mLStop Solution 1×6mLWash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mLInstruction manual 1Instruction manual 1
desc-type-1	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃; 8. Add 50µL Stop Solution. Read at 450nm immediately.
desc-insert-size-15:{813}(Description) Insert Size	12 months
prot-title-1	Yes
manual-link-4	This product is for research use only.
targ-symbol-1	E. coli
targ-subcellular-location-1	sandwich
targ-pathway-1	0.781-50ng/mL
targ-database-links-1	Colorimetric
targ-uniprot-1:{78}(Target) Uniprot	CE&ISO
prot-url-1	serum, plasma, tissue homogenates or other biological fluids.
desc-specificity-1	Quantitative

desc-insert-gene-species-15	P19468

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SKU	Supplier No.	Size	Your price	Quantity
BHE13502340		Traditional--96T	$747.26		Add to Cart
BHE13502340		Traditional--48T	$538.26		Add to Cart
BHE13502340		Ready-to-Use--96T	$728.35		Add to Cart
BHE13502340		Ready-to-Use--48T	$562.66		Add to Cart

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