It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. From 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined.
desc-cellular-localization-1
Immunohistology (Formalin-fixed)0.5-1μg/ml for 30 minutes at RT
desc-conjugate-1
P16150
desc-description-1:{15}(Description) Description
6693
desc-formulation-1:{18}(Description) Formulation
SPN, 2049R
immunogen-region
SPN
desc-intended-use-1
95, 115, or 135kDa
desc-involvement-in-disease-1
200μg/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype
1.0mg/ml
instructions-for-use
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
desc-positive-control-1
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody SPN,2049R Unconjugated BHA10208129