This MAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.ĀDeficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.
HeLa cells. Liver or Hepatocellular Carcinoma (HCC)
elisa-detection
Human, Dog
desc-reactivity-1
FALSE
desc-molecular-weight-1
RUO
desc-applications-8
IF, IHC-F, IHC(ap)
desc-categories-1
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
desc-cellular-localization-1
IF0.5-1.0μg/mlIHC-F0.25-0.5μg/ml for 30 minutes at RTIHC(ap)
desc-conjugate-1
P31327
desc-constituent-1
149252
desc-description-1:{15}(Description) Description
1373
desc-involvement-in-disease-1
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype
1.0mg/ml
instructions-for-use
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Reviews of Anti-CPS1 / Carbamoyl-Phosphate Synthetase Antibody BHA10200577