The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LRP5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)87-101%86-105%85-105%86-102%EDTA plasma(n=5)83-101%82-96%84-91%88-99%heparin plasma(n=5)81-97%83-99%82-96%80-95%
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Matrices listed below were spiked with certain level of LRP5 and the recovery rates were calculated by comparing the measured value to the expected amount of LRP5 in samples.MatrixRecovery range(%)Average(%)serum(n=5)89-10496EDTA plasma(n=5)90-10195heparin plasma(n=5)85-10396