Enter to Debug Area HSP90 alpha ELISA Kit

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HSP90 alpha ELISA Kit

SKU : BHE11900005

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StressMarq Biosciences Inc.

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desc-synonyms-1:{65}(Description) Synonyms	1. Prepare Standard and samples in Standard and Sample Diluent.2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour.4. Wash plate four times with 1X Wash Buffer.5. Add 100 µL of Biotinylated Antibody Working Solution to each well.6. Cover plate with Plate Sealer and incubate at room temperature, 20-25 °C for 1 hour.7. Wash plate four times with 1X Wash Buffer.8. Add 100 µL of Streptavidin-HRP Working Solution to each well.9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.10. Wash plate four times with 1X Wash Buffer.11. Add 100 µL of TMB Substrate to each well.12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well.14. Measure absorbance on a plate reader at 450 nm.
desc-bacterial-resistance-15	StressXpress®
desc-categories-1:{7}(Description) Categories	Human\|Goat
prop-gross-weight-kg-13	Hu\|Gt
refe-reference-1:{74}(References) Reference	0.117 ng/ml
prop-storage-stability-1:{113}(Property) Storage Stability	Microplate
prop-storage-condition-1	40 samples in duplicate
prop-dilutions-1	Anti-Hsp90a Immunoassay Plate\|5X Hsp90a Extraction Reagent\|Recombinant Hsp90a Standard\|Standard and Sample Diluent\|10X Wash Buffer Concentrate\|Anti-Hsp90a Biotinylated Antibody Concentrate\|Anti-Hsp90a Biotinylated Antibody Diluent\|Streptavidin: HRP Concentrate\|Streptavidin: HRP Diluent\|TMB Substrate\|Stop Solution
targ-weight-1	30 minutes
targ-pathway-1	0.44 - 28 ng/ml
targ-database-links-1	Colorimetric Assay
targ-target-1	Canada
targ-accession-number-1	SKC-107A\|SKC-107B\|SKC-107C\|SKC-107D\|SKC-107E\|SKC-107F\|SKC-107G\|SKC-107H\|SKC-107I\|SKC-107J\|SKC-107K
appl-dilution-1	Sandwich
appl-description-1	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
desc-usage-1	0.44 - 28 ng/ml
desc-species-reactivity-1	Non-hazardous
prop-storage-buffer-1	Cell Lysates, Tissue, Serum
prot-url-1	Cell Lysates, Tissue, Serum

desc-vector-type-15	ELISA kit used to quantitate HSP90 alpha concentration in samples.
desc-site-destroyed-during-cloning-15	Y

desc-mutation-15	12352203
desc-fusion-protein-or-tag-15	3822.00.1090

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SKU	Supplier No.	Size	Your price	Quantity
BHE11900005		96 well	$730.11		Add to Cart
BHE11900005		5 x 96 well	$2589.75		Add to Cart

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