Enter to Debug Area HSP70 ELISA Kit (High-Sensitivity)

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Home / Assay Kits

HSP70 ELISA Kit (High-Sensitivity)

SKU : BHE11900007

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StressMarq Biosciences Inc.

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desc-synonyms-1:{65}(Description) Synonyms	1. Prepare Standard and samples in Standard and Sample Diluent.2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 2 hours.4. Wash plate four times with 1X Wash Buffer.5. Add 100 µL of Biotinylated Antibody Working Solution to each well.6. Cover plate with Plate Sealer and incubate at at 37°C for 2 hours.7. Wash plate four times with 1X Wash Buffer.8. Add 100 µL of Streptavidin-HRP Working Solution to each well.9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes.10. Wash plate four times with 1X Wash Buffer.11. Add 100 µL of TMB Substrate to each well.12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well.14. Measure absorbance on a plate reader at 450 nm.
desc-bacterial-resistance-15	StressXpress®
desc-categories-1:{7}(Description) Categories	Human\|Monkey\|Dog\|Rat\|Mouse
prop-gross-weight-kg-13	Hu\|Mk\|Dg\|Rt\|Ms
refe-reference-1:{74}(References) Reference	0.02 ng/ml
prop-storage-stability-1:{113}(Property) Storage Stability	Microplate
prop-storage-condition-1	40 samples in duplicate
desc-species-reactivity-1	Non-hazardous
desc-usage-1	0.55 - 35 ng/ml
appl-description-1	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
prop-dilutions-1	Anti-Hsp70 Immunoassay Plate\|Recombinant Hsp70 Standard\|Standard and Sample Diluent\|10X Wash Buffer Concentrate\|Anti-Hsp70 Biotinylated Antibody Concentrate\|Anti-Hsp70 Biotinylated Antibody Diluent\|Streptavidin: HRP Concentrate\|Streptavidin: HRP Diluent\|TMB Substrate\|Stop Solution
targ-weight-1	30 minutes
appl-dilution-1	Sandwich
targ-accession-number-1	SKC-108A\|SKC-108B\|SKC-108C\|SKC-108D\|SKC-108E\|SKC-108F\|SKC-108G\|SKC-108H\|SKC-108I\|SKC-108J
targ-target-1	Canada
targ-database-links-1	Colorimetric Assay
targ-pathway-1	0.55 - 35 ng/ml
prot-url-1	Tissue, Cell Lysates, Plasma, Serum
prop-storage-buffer-1	Serum, Tissue, Cell Lysates, Plasma

desc-vector-type-15	ELISA kit used to quantitate HSP70 concentration in samples.
desc-site-destroyed-during-cloning-15	Y

desc-mutation-15	12352203
desc-fusion-protein-or-tag-15	3822.00.1090

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SKU	Supplier No.	Size	Your price	Quantity
BHE11900007		96 well	$730.11		Add to Cart
BHE11900007		5 x 96 well	$2589.75		Add to Cart

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