The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSD3B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)86-102%87-101%86-104%86-100%EDTA plasma(n=5)85-95%91-100%82-94%90-99%heparin plasma(n=5)84-97%80-97%86-100%85-93%
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Matrices listed below were spiked with certain level of HSD3B1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSD3B1 in samples.MatrixRecovery range(%)Average(%)serum(n=5)86-10393EDTA plasma(n=5)92-10198heparin plasma(n=5)95-10399