Enter to Debug Area Collagen Type IV Alpha 1 ELISA Kit BHE10101110

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Collagen Type IV Alpha 1 ELISA Kit BHE10101110

SKU : BHE10101110

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appl-description-1	Double-antibody Sandwich
targ-gene-id-1	Reagents Quantity Reagents QuantityPre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4Standard 2 Standard Diluent 1×20mLDetection Reagent A 1×120µL Assay Diluent A 1×12mLDetection Reagent B 1×120µL Assay Diluent B 1×12mLTMB Substrate 1×9mL Stop Solution 1×6mLWash Buffer (30 × concentrate) 1×20mL Instruction manual 1
targ-pathway-1	4.7-300ng/mL
prop-purity-1	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type IV Alpha 1 (COL4a1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1:21:41:81:16serum(n=5)91-102%91-105%83-99%91-102%EDTA plasma(n=5)83-93%91-98%98-105%95-102%heparin plasma(n=5)89-105%86-97%81-89%78-91%
appl-applications-1	3h
refe-reference-1:{74}(References) Reference	< 1.9ng/mL
manual-link-0:{925}(Protocols) Product Manual	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
manual-link-3	Matrices listed below were spiked with certain level of recombinant Collagen Type IV Alpha 1 (COL4a1) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type IV Alpha 1 (COL4a1) in samples.MatrixRecovery range (%)Average(%)serum(n=5)89-10394EDTA plasma(n=5)86-10295heparin plasma(n=5)90-10196
prot-url-1	Biological Fluids, Cell Culture Supernates, Cell Lysates, Tissue Homogenates, Plasma, Serum
prop-storage-buffer-1	Biological Fluids, Cell Culture Supernates, Cell Lysates, Tissue Homogenates, Plasma, Serum

desc-gene-alt-name-15	Homo sapiens (Human)

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SKU	Supplier No.	Size	Your price	Quantity
BHE10101110		96T	$598.50		Add to Cart

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