The Blood Urea Nitrogen (BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14.
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StressXpress®
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Species Independent
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ALL
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0.030 mg/dl
manual-link-0:{925}(Protocols) Product Manual
Inter Assay Precision: Three human samples were further diluted in water and run in duplicates in twenty-eight assays run over multiple days by five operators. The mean and precision of the calculated concentrations were:Sample 1- 1.29 mg/dL, 3.1% CVSample 2- 2.35 mg/dL, 4.3% CVSample 3- 5.18 mg/dL, 3.3% CV