Enter to Debug Area Human Frizzled Homolog 1 (FZD1) ELISA Kit

JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits

Human Frizzled Homolog 1 (FZD1) ELISA Kit

SKU : BHE13502259

test cart View all offers

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

Wuxi Donglin Sci & Tech Development Co.,Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

targ-target-details-1	The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.
desc-backbone-size-w-o-insert-15	The microtiter plate provided in this kit has been pre-coated with an antibody specific to FZD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FZD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FZD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FZD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
desc-5-cloning-site-15	Traditional ELISA KitReady-to-Use ELISA KITProduct name:Human Frizzled Homolog 1 (FZD1) ELISA KitMethod: SandwichSynonyms:FzE1; Wnt ReceptorDetection range:0.156-10ng/mLReactivity: FZD1Size:96T/48TQuality guarantee period:for 12 monthsCatalog number:DL-FZD1-Hu(traditional)DLR-FZD1-Hu(ready-to-use)Assay length 1-4.5Hours 1-3.5HoursAdvantages:Competitive price.High sensitivity.High stability.12 months shelf life.Pre-diluted Detection Reagent A and BReduction in the number of steps when conducting the testAll the reagents can be stored at 4℃Faster reaction compare to other brands12 months shelf lifeInstruction Manual
manual-link-3	Matrices listed below were spiked with certain level of recombinant FZD1 and the recovery rates were calculated by comparing the measured value to the expected amount of FZD1 in samples.MatrixRecovery range (%)Average(%)serum(n=5)84-9991EDTA plasma(n=5)82-10392heparin plasma(n=5)84-10293
manual-link-0:{925}(Protocols) Product Manual	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100Intra-assay: CV
prop-purity-1	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FZD1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1：21：41：81：16serum(n=5)82-96%83-98%81-99%93-101%EDTA plasma(n=5)88-101%86-95%90-102%80-93%heparin plasma(n=5)80-91%82-90%95-104%79-95%
prop-description-1	ItemStandardTestDescription The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FZD1 in human tissue homogenates or other biological fluids.ConformIdentificationColorimetricPositiveCompositionTraditional ELISA KitReady-to-Use ELISA KITConformPre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1Plate sealer for 96 wells 2Plate sealer for 96 wells 2Standard 2Standard 2Diluents buffer 1×45mLStandard Diluent 1×20mLDetection Reagent A 1×120μLDetection Solution A 1×12mLDetection Reagent B 1×120μLDetection Solution B 1×12mLTMB Substrate 1×9mLTMB Substrate 1×9mLStop Solution 1×6mLStop Solution 1×6mLWash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mLInstruction manual 1Instruction manual 1
desc-type-1	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃; 8. Add 50µL Stop Solution. Read at 450nm immediately.
desc-insert-size-15:{813}(Description) Insert Size	12 months
prot-title-1	Yes
manual-link-4	This product is for research use only.
targ-symbol-1	E. coli
targ-subcellular-location-1	sandwich
targ-pathway-1	0.156-10ng/mL
targ-database-links-1	Colorimetric
targ-uniprot-1:{78}(Target) Uniprot	CE&ISO
prot-url-1	tissue homogenates or other biological fluids.
desc-specificity-1	Quantitative

desc-insert-gene-species-15	Q9UP38

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13502259		Traditional--96T	$697.26		Add to Cart
BHE13502259		Traditional--48T	$503.26		Add to Cart
BHE13502259		Ready-to-Use--96T	$729.56		Add to Cart
BHE13502259		Ready-to-Use--48T	$525.86		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

Who We are
Product Type
Select Services
- Antibody Service
- Custome Services
HELP & SUPPORT

© 2026 ebiohippo. All rights reserved.