Enter to Debug Area General Histamine (HIS) ELISA Kit

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Home / Assay Kits

General Histamine (HIS) ELISA Kit

SKU : BHE13502708

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Wuxi Donglin Sci & Tech Development Co.,Ltd.

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Description
Review(s)

prot-title-1	Yes
desc-backbone-size-w-o-insert-15	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to HIS has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled HIS and unlabeled HIS (Standards or samples) with the pre-coated antibody specific to HIS. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of HIS in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of HIS in the sample.
desc-5-cloning-site-15	Traditional ELISA KitReady-to-Use ELISA KITProduct name:General Histamine (HIS) ELISA KitMethod: CompetitionSynonyms:HistaminDetection range:3.12-200ng/mLReactivity: HISSize:96T/48TQuality guarantee period:for 12 monthsCatalog number:DL-HIS-Ge(traditional)DLR-HIS-Ge(ready-to-use)Assay length 1-2.5Hours 1-2.5HoursAdvantages:Competitive price.High sensitivity.High stability.12 months shelf life.Pre-diluted Detection Reagent A and BReduction in the number of steps when conducting the testAll the reagents can be stored at 4℃Faster reaction compare to other brands12 months shelf lifeInstruction Manual
manual-link-3	Matrices listed below were spiked with certain level of recombinant HIS and the recovery rates were calculated by comparing the measured value to the expected amount of HIS in samples.MatrixRecovery range (%)Average(%)serum(n=5)86-10093EDTA plasma(n=5)87-10093heparin plasma(n=5)88-10094
manual-link-0:{925}(Protocols) Product Manual	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100Intra-assay: CV
prop-purity-1	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1：21：41：81：16serum(n=5)82-96%83-98%81-99%93-101%EDTA plasma(n=5)88-101%86-95%90-102%80-93%heparin plasma(n=5)80-91%82-90%95-104%79-95%
prop-description-1	ItemStandardTestDescription The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of HIS in serum, plasma, tissue homogenates and other biological fluids.ConformIdentificationColorimetricPositiveCompositionTraditional ELISA KitReady-to-Use ELISA KITConformPre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1Plate sealer for 96 wells 2Plate sealer for 96 wells 2Standard 2Standard 2Diluents buffer 1×45mLStandard Diluent 1×20mLDetection Reagent A 1×120μLDetection Solution A 1×12mLDetection Reagent B 1×120μLDetection Solution B 1×12mLTMB Substrate 1×9mLTMB Substrate 1×9mLStop Solution 1×6mLStop Solution 1×6mLWash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mLInstruction manual 1Instruction manual 1
desc-insert-size-15:{813}(Description) Insert Size	12 months
manual-link-4	This product is for research use only.
targ-symbol-1	E. coli
targ-subcellular-location-1	competitive inhibition
targ-pathway-1	3.12-200ng/mL
targ-database-links-1	Colorimetric
targ-uniprot-1:{78}(Target) Uniprot	CE&ISO
targ-target-details-1	The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.
desc-specificity-1	Quantitative
prot-url-1	serum, plasma, tissue homogenates or other biological fluids.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13502708		Traditional--96T	$723.94		Add to Cart
BHE13502708		Traditional--48T	$559.16		Add to Cart
BHE13502708		Ready-to-Use--96T	$756.61		Add to Cart
BHE13502708		Ready-to-Use--48T	$584.56		Add to Cart

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