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Bovine Growth Hormone Releasing Hormone (GHRH) ELISA Kit

SKU : BHE13502401

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Wuxi Donglin Sci & Tech Development Co.,Ltd.

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targ-target-details-1	The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this reagent.
desc-backbone-size-w-o-insert-15	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GHRH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GHRH and unlabeled GHRH (Standards or samples) with the pre-coated antibody specific to GHRH. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GHRH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GHRH in the sample.
desc-5-cloning-site-15	Traditional ELISA KitReady-to-Use ELISA KITProduct name:Bovine Growth Hormone Releasing Hormone (GHRH) ELISA KitMethod: CompetitionSynonyms:GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; SomatoliberinDetection range:15.625-1000pg/mLReactivity:GHRHSize:96T/48TQuality guarantee period:for 12 monthsCatalog number:DL-GHRH-b(traditional)DLR-GHRH-b(ready-to-use)Assay length 1-2.5Hours 1-2.5HoursAdvantages:Competitive price.High sensitivity.High stability.12 months shelf life.Pre-diluted Detection Reagent A and BReduction in the number of steps when conducting the testAll the reagents can be stored at 4℃Faster reaction compare to other brands12 months shelf lifeInstruction Manual
manual-link-3	Matrices listed below were spiked with certain level of recombinant GHRH and the recovery rates were calculated by comparing the measured value to the expected amount of GHRH in samples.MatrixRecovery range (%)Average(%)serum(n=5)85-10193EDTA plasma(n=5)86-9892heparin plasma(n=5)87-10194
manual-link-0:{925}(Protocols) Product Manual	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100Intra-assay: CV
prop-purity-1	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GHRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.Sample1：21：41：81：16serum(n=5)82-96%83-98%81-99%93-101%EDTA plasma(n=5)88-101%86-95%90-102%80-93%heparin plasma(n=5)80-91%82-90%95-104%79-95%
prop-description-1	ItemStandardTestDescription The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GHRH in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.ConformIdentificationColorimetricPositiveCompositionTraditional ELISA KitReady-to-Use ELISA KITConformPre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1Plate sealer for 96 wells 2Plate sealer for 96 wells 2Standard 2Standard 2Diluents buffer 1×45mLStandard Diluent 1×20mLDetection Reagent A 1×120μLDetection Solution A 1×12mLDetection Reagent B 1×120μLDetection Solution B 1×12mLTMB Substrate 1×9mLTMB Substrate 1×9mLStop Solution 1×6mLStop Solution 1×6mLWash Buffer (30 × concentrate) 1×20mLWash Buffer (30 × concentrate) 1×20mLInstruction manual 1Instruction manual 1
desc-insert-size-15:{813}(Description) Insert Size	12 months
prot-title-1	Yes
manual-link-4	This product is for research use only.
targ-symbol-1	E. coli
targ-subcellular-location-1	competitive inhibition
targ-pathway-1	15.6-1000pg/mL
targ-database-links-1	Colorimetric
targ-uniprot-1:{78}(Target) Uniprot	CE&ISO
prot-url-1	serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
desc-specificity-1	Quantitative

desc-insert-gene-species-15	P63292

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SKU	Supplier No.	Size	Your price	Quantity
BHE13502401		Traditional--96T	$743.86		Add to Cart
BHE13502401		Traditional--48T	$535.86		Add to Cart
BHE13502401		Ready-to-Use--96T	$725.20		Add to Cart
BHE13502401		Ready-to-Use--48T	$560.16		Add to Cart

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