Anti-EMI1 (Early Mitotic Inhibitor-1) Antibody BHA10201424

desc-alternative-names-1:(Description) Alternative Names	EMI1 (Early Mitotic Inhibitor-1)
desc-antigen-1:(Description) Antigen	Early Mitotic Inhibitor-1 (EMI1)\|FBX5\|Fbxo31
desc-specificity-1:(Description) Specificity	Nuclear
prop-purity-1:(Property) Purity	6q25.2
prop-storage-1:(Property) Storage	monoclonal
targ-uniprot-1	It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
desc-host-1:{21}(Product Details) Host	Mouse
prop-purification-1:{101}(Product Details) Purification	Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
appl-tested-application-1:{73}(Product Details) Tested Application	IgG2a Kappa
desc-assay-procedure-3	HeLa, 293 or HepG2 cells. Ovarian carcinoma.
elisa-detection	Human
desc-reactivity-1	FALSE
desc-molecular-weight-1	RUO

desc-applications-8	FACS, IHC-F, WB, IF
desc-categories-1	(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
desc-cellular-localization-1	FACS0.5-1μg/million cells in 0.1mlIF0.5-1μg/mlWB1-2μg/ml for 60 minutes at RTIHC-F0.5-1μg/ml for 30 minutes at RT

desc-conjugate-1	Q9UKT4
desc-constituent-1	520506
desc-description-1:{15}(Description) Description	26271

desc-involvement-in-disease-1	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype	1.0mg/ml
instructions-for-use	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml