Anti-Chk1 Antibody (Chk1S317-G1) Unconjugated BHA10600040

Mfr.No.	2261
desc-alternative-names-1:(Description) Alternative Names	Chk1
prop-storage-1:(Property) Storage	monoclonal
prop-storage-buffer-1:(Property) Storage Buffer	Unconjugated
targ-uniprot-1	The act of DNA damaged response and cell cycle checkpoints requires the activation of four protein kinases that form the canonical ATR-Chk1 and ATM-Chk2 pathways. ATR activation requires the generation of structures containing single strand DNA (ssDNA) adjacent to double strand DNA (dsDNA). Such ssDNA is coated with replication protein A complex and attracts ATR (1,2). The accumulation of ATR to damage sites results in initial activation of ATR. ATR phosphorylates proteins at the ssDNA which are called checkpoint regulators. The accumulation and phosphorylation of these checkpoint regulators further stimulates the catalytic activity of ATR. ATR-induced Chk1 phosphorylation likely occurs at the sites of DNA damage on chromatin (3-5). The activated ATR phosphorylates Chk1 at Ser317 and Ser345 in its C-terminal regulatory domain. Phospho Chk1 is critical for DNA damage checkpoint activation, replication control, and cell viability (6-8). Functionally, ATR-mediated phosphorylation elevates phospho Chk1 catalytic activity. The N-terminal catalytic domain of Chk1 adopts an open kinase conformation and the deletion of C-terminal domain increases Chk1 catalytic activity.
desc-host-1:{21}(Product Details) Host	Mouse
prop-purification-1:{101}(Product Details) Purification	A synthetic phospho-peptide corresponding to residues surrounding Ser317 of human phospho Chk1
appl-tested-application-1:{73}(Product Details) Tested Application	IgG1 Kappa
standard-form	phospho-Ser317
elisa-detection	Mouse, Human
desc-sample-type-3	RUO

desc-applications-8	WB, FACS

desc-formulation-1:{18}(Description) Formulation	Chk1S317-G1

desc-involvement-in-disease-1	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
desc-isotype-1:{29}(Description) Isotype	0.5mg/ml
biological-activity	1.0 - 0.1 µg/ml
desc-positive-control-1	-20ºC