This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.
MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
elisa-detection
Human
desc-reactivity-1
TRUE
desc-molecular-weight-1
RUO
desc-applications-8
IHC-F
desc-categories-1
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined.
desc-cellular-localization-1
Immunohistology (Formalin-fixed)0.5-1μg/ml for 30 minutes at RT
desc-conjugate-1
P15941
desc-description-1:{15}(Description) Description
4582
desc-formulation-1:{18}(Description) Formulation
MUC1, 2278R
immunogen-region
MUC1
desc-intended-use-1
265-400kDa
desc-involvement-in-disease-1
200μg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype
1.0mg/ml
instructions-for-use
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
desc-positive-control-1
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
No Description Available.
No Description Available.
Reviews of Anti-undefined Antibody MUC1,2278R Unconjugated BHA10208254