Enter to Debug Area Anti-undefined Antibody AKT1,2491 Unconjugated BHA10207806

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Home / Antibodies

Anti-undefined Antibody AKT1,2491 Unconjugated BHA10207806

SKU : BHA10207806

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NeoBiotechnologies, Inc.

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desc-alternative-names-1:(Description) Alternative Names	AKT1 (Proto-Oncogene)
desc-antigen-1:(Description) Antigen	AKT1\|Oncogene AKT1\|PKB\|PKB-ALPHA\|PRKBA\|Protein Kinase B Alpha\|Proto-oncogene c-Akt\|RAC Alpha\|RAC Serine/Threonine Protein Kinase\|RAC-alpha serine/threonine-protein kinase\|RAC-PK-alpha
desc-specificity-1:(Description) Specificity	Nuclear, Cytoplasmic, Cell Surface
prop-purity-1:(Property) Purity	14q32.32
prop-storage-1:(Property) Storage	monoclonal
prop-storage-buffer-1:(Property) Storage Buffer	Unconjugated
targ-uniprot-1	Recognizes a protein of 62kDa, which is identified as AKT1The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB? or RacPK-?) and Akt 3 (also designated PKB? or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-? tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
desc-host-1:{21}(Product Details) Host	Mouse
prop-purification-1:{101}(Product Details) Purification	Recombinant human AKT1 protein fragment (around aa 85-189) (exact sequence is proprietary)
appl-tested-application-1:{73}(Product Details) Tested Application	IgG2b Kappa
desc-assay-procedure-3	PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.
elisa-detection	Human
desc-reactivity-1	FALSE
desc-molecular-weight-1	RUO
desc-alternative-names-1	Homo Sapiens (Human)

desc-applications-8	IHC-F
desc-categories-1	Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes,Optimal dilution for a specific application should be determined.
desc-cellular-localization-1	Immunohistology (Formalin-fixed)1-2μg/ml for 30 min at RT

desc-conjugate-1	P31749
desc-description-1:{15}(Description) Description	207
desc-formulation-1:{18}(Description) Formulation	AKT1, 2491
immunogen-region	AKT1
desc-intended-use-1	62kDa

desc-involvement-in-disease-1	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype	200μg/ml
instructions-for-use	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1	Purified Ab with BSA and Azide at 200μg/ml
desc-positive-control-1	Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207806		20µg	$204.67		Add to Cart
BHA10207806		100µg	$393.20		Add to Cart

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