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Home / Antibodies

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205392

SKU : BHA10205392

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NeoBiotechnologies, Inc.

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desc-alternative-names-1:(Description) Alternative Names	TNF-alpha (Tumor Necrosis Factor alpha)
desc-antigen-1:(Description) Antigen	APC1\|Cachectin\|Differentiation inducing factor (DIF)\|Macrophage cytotoxic factor (MCF)\|Necrosin\|TNF alpha\|TNF Macrophage Derived\|TNF Monocyte Derived\|TNF Superfamily Member 2\|TNFA
desc-specificity-1:(Description) Specificity	Cytoplasmic, Extracellular(secreted)
prop-purity-1:(Property) Purity	6p21.33
prop-storage-1:(Property) Storage	monoclonal
targ-uniprot-1	This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
desc-host-1:{21}(Product Details) Host	Mouse
prop-purification-1:{101}(Product Details) Purification	Recombinant human TNF-alpha
appl-tested-application-1:{73}(Product Details) Tested Application	IgG1 Kappa
desc-assay-procedure-3	HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
elisa-detection	Human
desc-reactivity-1	FALSE
desc-molecular-weight-1	RUO

desc-applications-8	FACS, IF
desc-categories-1	Inhibits Tumor Growth (Order Ab without BSA/Azide); Neutralizes rTNF-alpha Mediated Cytotoxicity (Order Ab without BSA/Azide); Protects Mice Against Toxicity of rhTNF-alpha (Order Ab without BSA/Azide); Optimal dilution for a specific application should be determined.
desc-cellular-localization-1	FACS0.5-1μg/million cells in 0.1mlIF1-2μg/ml

desc-conjugate-1	P01375
desc-constituent-1	241570
desc-description-1:{15}(Description) Description	7124

desc-involvement-in-disease-1	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype	1.0mg/ml
instructions-for-use	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205392		100µg	$393.20		Add to Cart

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