Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205372

desc-alternative-names-1:(Description) Alternative Names	TNF-alpha (Tumor Necrosis Factor alpha)
desc-antigen-1:(Description) Antigen	APC1\|Cachectin\|Differentiation inducing factor (DIF)\|Macrophage cytotoxic factor (MCF)\|Necrosin\|TNF alpha\|TNF Macrophage Derived\|TNF Monocyte Derived\|TNF Superfamily Member 2\|TNFA
desc-specificity-1:(Description) Specificity	Cytoplasmic, Extracellular(secreted)
prop-purity-1:(Property) Purity	6p21.33
prop-storage-1:(Property) Storage	monoclonal
targ-uniprot-1	This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
desc-host-1:{21}(Product Details) Host	Mouse
prop-purification-1:{101}(Product Details) Purification	Recombinant full-length human TNF-alpha protein
appl-tested-application-1:{73}(Product Details) Tested Application	IgM Kappa
desc-assay-procedure-3	HeLa, HL-60, or A431 cells. Pancreas or Histiocytoma
elisa-detection	Human, Rat
desc-reactivity-1	FALSE
desc-molecular-weight-1	RUO

desc-applications-8	FACS, IF, IHC-FP
desc-categories-1	(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
desc-cellular-localization-1	FACS0.5-1μg/million cells in 0.1mlIF1-2μg/mlIHC-FP2-4μg/ml for 30 min at RT

desc-conjugate-1	P01375
desc-constituent-1	241570
desc-description-1:{15}(Description) Description	7124

desc-involvement-in-disease-1	200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype	1.0mg/ml
instructions-for-use	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml