Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
elisa-detection
Human, Monkey, Mouse, Rat
desc-reactivity-1
FALSE
desc-molecular-weight-1
RUO
desc-applications-8
FACS, IF, WB, IHC-F
desc-categories-1
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
desc-cellular-localization-1
FACS0.5-1μg/million cells in 0.1mlIF0.5-1μg/mlWB0.5-1μg/mlIHC-F0.25-0.5μg/ml for 30 minutes at RT
desc-conjugate-1
P46527
desc-constituent-1
238990
desc-description-1:{15}(Description) Description
1027
desc-involvement-in-disease-1
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype
1.0mg/ml
instructions-for-use
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Reviews of Anti-p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Antibody BHA10200113