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Home / Antibodies

Anti-Glypican-3 (GPC3) Antibody BHA10201521

SKU : BHA10201521

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NeoBiotechnologies, Inc.

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desc-alternative-names-1:(Description) Alternative Names	Glypican-3 (GPC3)
desc-antigen-1:(Description) Antigen	DGSX\|Glypican proteoglycan 3\|GPC3\|GTR2-2\|Heparan sulphate proteoglycan\|Intestinal protein OCI-5\|MXR7\|OCI-5\|SDYS\|Secreted glypican-3
desc-specificity-1:(Description) Specificity	Cytoplasmic
prop-purity-1:(Property) Purity	Xq26.2
prop-storage-1:(Property) Storage	monoclonal
targ-uniprot-1	Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
desc-host-1:{21}(Product Details) Host	Mouse
prop-purification-1:{101}(Product Details) Purification	A recombinant fragment containing amino acids 511-580 of human glypican-3
appl-tested-application-1:{73}(Product Details) Tested Application	IgG1 Kappa
desc-assay-procedure-3	293T cells or Hepatocellular carcinoma
elisa-detection	Human
desc-reactivity-1	FALSE
desc-molecular-weight-1	RUO

desc-applications-8	FACS, IHC(ap), IHC-F, IF
desc-categories-1	(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
desc-cellular-localization-1	FACS0.5-1μg/million cells in 0.1mlIF0.5-1μg/mlIHC-F0.5-1μg/ml for 30 minutes at RTIHC(ap)

desc-conjugate-1	P51654
desc-constituent-1	644108
desc-description-1:{15}(Description) Description	2719

desc-involvement-in-disease-1	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
desc-isotype-1:{29}(Description) Isotype	1.0mg/ml
instructions-for-use	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
desc-net-weight-g-1	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10201521		100µg	$393.20		Add to Cart

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